A multilocus phylogeny of the streptomyces griseus 16s. Multilocus sequence typing mlst is an unambiguous procedure for characterising isolates of bacterial species using the sequences of internal fragments of usually seven housekeeping genes. A multilocus phylogeny of the streptomyces griseus 16s rrna gene clade. For many species, multilocus sequence typing mlst is considered the gold standard of typing, but it is traditionally performed in an expensive and timeconsuming manner. Classical mlst schemes typically define seven loci housekeeping genes. Unique sequences for each locus are assigned allele numbers and bacterial strains are identified based on their allelic profiles, which is the combination of the seven allele. One major contribution of mlst to bacterial multilocus sequence typing of pathogens 515 population genetics has been the elucidation of the relative role of recombination and point mutation in the evolutionary history of different bacterial species. Accurate strain identification is essential for anyone working with bacteria. Multilocus sequence typing mlst was proposed in 1998 as a portable, universal, and definitive method for characterizing bacteria, using the human pathogen as an example. Pdf multilocus sequence typing of totalgenomesequenced. Approximately 450500 bp internal fragments of each gene are used, as these can be accurately sequenced on both strands using an automated dna sequencer.